Fig. 5

Western blotting analysis of proteins related to apoptosis in T24 and 5637 cells. a was showed AKT, p-AKT, PI3K, mTOR, Caspase3 and Bcl-2 expression in T24 and 5637 cells measured by immunoblotting. And β-actin was used as an internal control. b and c were showed the relative protein levels normalized to β-actin in T24 and 5637 cells, including Con, NC and Maspin group, respectively. AKT expression did’t have significant changes between each three group (P > 0.05). p-AKT, PI3K, mTOR, and Bcl-2 expression were significantly decreased, whereas Caspase3 was greatly increased in the Maspin group. *P < 0.05. Three independent experiments were performed (n = 3)