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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells

Fig. 3

Over-expression of miR-208a promoted cellular proliferation. a The effect of miR-208a on viability of the A549 cells was analyzed using the MTT assay, and the miRNA-NC-transfected cells were normalized as 100 %. b The proliferating A549 cells were labeled with EdU. The click-it reaction revealed the EdU staining (red). The cell nuclei were stained with Hoechst 33342 (blue). The images are representative of the results obtained. c Colony formation assays of A549 cells transfected with miR-208a mimics or miR-208a inhibitor. One thousand cells were seeded onto each plate. After 10 days, the cells were stained with crystal violet. The colonies consisting of more than 50 cells were counted. The data are presented as the means ± SEM (n = 4). *P < 0.05 and **P < 0.01 compared with the negative control

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