Fig. 4

miR-208a increased the cell apoptosis and disturbed the cell cycle of lung cancer cells. a A549 and (b) H1975 cells were transfected with the indicated vectors. Two days after transfection, the percentage of apoptotic cells was evaluated using flow cytometry. Western blot analyzed the apoptotic molecular component including PARP1, Bcl2 and Bax in (c) A549 and (d) H1975 cells. The cell cycle distributions of (e) A549 and (f) H1975 cells were measured using flow cytometry. *P < 0.05 and **P < 0.01 compared with the negative control