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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: The human RNA surveillance factor UPF1 regulates tumorigenesis by targeting Smad7 in hepatocellular carcinoma

Fig. 7

UPF1 affected the TGF-β pathway by targeting Smad7. a The expression of UPF1 and Smad7 protein was illustrated in paired tumor tissues and non-tumor tissues from patients with HCC. N indicates adjacent normal tissue; T, tumor tissue. (b) Bivariate correlation analysis of the negative association between UPF1 mRNA expression level and Smad7 mRNA expression level in 50 HCC tissues. *p < 0.05. (c, e) The UPF1 and Smad7 protein expression levels in HCC cells were examined using western blotting and immunofluorescence when UPF1 was silenced. *p < 0.05; **p < 0.01, (Scale bars =20 μm). d The UPF1 and Smad7 protein expression levels in HCC cells were examined using western blotting when UPF1 was overexpressed. *p < 0.05; **p < 0.01. f The expression and phosphorylation of key molecules of the TGF-β pathways (Smad2, Smad3) were detected using western blotting. GAPDH was used as an internal control. *p < 0.05

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