Fig. 1

MiR-224 activates the Wnt/β-catenin signaling and results in subsequent β-catenin relocation. a TOP/FOP Luciferase assay revealed that the activity of Wnt/β-catenin signaling pathway positively correlated with miR-224 expression. b Western blot analysis of crucial downstream molecules and (c) real-time PCR analysis of crucial downstream molecules. d β-catenin expression was assessed by immunofluorescence staining in both cell lines treated with the negative control miRNA or miR-224 mimics or miR-224 inhibitors for 48 h. β-catenin is shown in red, and the nuclei are counterstained with DAPI that are shown in blue. Scale bars = 10 μm. Error bars represent mean ± SD from 3 independent experiments; * p < 0.05, ** p < 0.01