Fig. 2
From: MicroRNA-224 sustains Wnt/β-catenin signaling and promotes aggressive phenotype of colorectal cancer
MiR-224 could target GSK3β and SFRP2 in CRC. a Predicted miR-224 target sequences in the 3’-UTRs of GSK3β or SFRP2, and their mutants containing altered nucleotides in the 3’-UTRs. b Real-time PCR analysis of GSK3β or SFRP2. c Western blot analysis of GSK3β or SFRP2. d Luciferase assay analyses of the indicated cells transfected with the indicated reporters with increasing amounts of miR-224 (20 and 50 nM). Error bars represent mean ± SD from three independent experiments; * p < 0.05, ** p < 0.01