Fig. 3
From: MicroRNA-224 sustains Wnt/β-catenin signaling and promotes aggressive phenotype of colorectal cancer
MiR-224 manipulates the colon cancer cells proliferation and migration partly through GSK3β and SFRP2. a Western blot analysis of GSK3β or SFRP2. b Soft agar assay. Representative micrographs are shown (left) and only cell colonies > 0.1 mm in diameter were counted (right). c Representative images (left) and quantification (right) of migrated cells across a Transwell chamber. d Representative images (left) and quantification (right) of invaded cells across a Transwell chamber. e Representative micrographs of indicated cultured cells at day 7 of culture in three-dimensional morphogenesis assay. Error bars represent mean ± SD from 3 independent experiments; *p < 0.05, ** p < 0.01