Fig. 3

Inhibition of SPAG9 or upregulation of miR-141 reduces proliferation, migration, and invasion of HCC cells. a Western blot analysis of SPAG9 expression in LM3 and Huh7 cells transfected with SPAG9 siRNA (si-SPAG9) or control siRNA (si-Ctrl). b MTT assay showed that the inhibition of SPAG9 or ectopic expression of miR-141 could hinder Hep3B and Huh7 cell proliferation. c and d Wound-healing assay showed that SPAG9 depletion or miR-141 upregulation decreased cell migration of Huh7 cells. e and f Matrigel invasion assay showed that SPAG9 depletion or miR-141 upregulation decreased cell invasion of Huh7 cells. Data are presented as the mean ± S.D. (*P < 0.05, n = 3). U.T: untreated Huh7 cells