Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Direct targeting sperm-associated antigen 9 by miR-141 influences hepatocellular carcinoma cell growth and metastasis via JNK pathway

Fig. 5

MiR-141 regulates JNK signaling pathway via SPAG9 in HCC cells. a Western blot analysis showed that SPAG9 depletion or miR-141 transfection decreased JNK protein expression and downregulated JNK phosphorylation in Huh7 cells. b Western blot analysis showed that SPAG9 overexpression significantly restored the expression of phosphorylated JNK in miR-141-transfected Huh7 cells. c and d Real time-PCR (c) and Western blot analysis (d) of MMP9 and c-Jun expression in Huh7 cells transfected with miR-141 or co-transfected with miR-141 and SPAG9 plasmid. Data are presented as the mean ± S.D. (*P < 0.05, n = 3). U.T: untreated Huh7 cells; miR-141 + Ctrl: miR-141 and control vector co-transfected Huh7 cells; miR-141 + SPAG9: miR-141 and SPAG9 plasmid co-transfected Huh7 cells

Back to article page