Fig. 5

Tumoral angiogenesis and cell proliferation in situ. a Illustrated are representative sections prepared from tumors in Fig. 4b. Sections were stained with antibodies against NRP-1 (left panel), Ki67 (middle panel) or CD31 (right panel). The cell proliferation index (b) and microvessel density (c) were quantified. “n” indicates the number of tumors examined. d Homogenates prepared from tumors in Fig. 4b were immunoblotted. The density of each band was measured and normalized to that of β-actin. “*” (P < 0.05) and “**” (P < 0.001) indicate a significant difference from MGC-803 tumors