Fig. 2
HOXA1 expression in cell lines and the effects of HOXA1 knockdown in GC cell lines. a HOXA1 protein levels in five GC cell lines and b grayscale values were evaluated. SGC-7901 and BGC-823 showed higher HOXA1 expression than the other tested cell lines. c Western blotting analysis of HOXA1 expression in stable HOXA1 knockdown SGC-7901 and BGC-823 cell lines. d Real-time PCR analysis of HOXA1 expression in stable HOXA1 knockdown SGC-7901 and BGC-823 cell lines. e Effect of HOXA1 knockdown on cell growth was evaluated by the Cell Counting Kit-8 assay. Cell growth was inhibited by HOXA1 knockdown. f A plate colony formation assay was performed to assess the impact of HOXA1 knockdown on cell growth. HOXA1 knocked down GC cells showed a weaker clone formation. g The number of GC cell clones was quantified. (**P < 0.01, ***P < 0.001)