Fig. 2From: Eliciting cytotoxic T lymphocytes against human laryngeal cancer-derived antigens: evaluation of dendritic cells pulsed with a heat-treated tumor lysate and other antigen-loading strategies for dendritic-cell-based vaccinationPhenotypes of DCs loaded with SNU899-derived Ags by different methods. To induce maturation, control DCs and SNU899-derived Ag-loaded DCs were treated with 1 μg/ml LPS for 48 h. A DC maturation marker (CD83), MHC-II molecule (HLA-DR), and co-stimulatory molecules (CD80, CD86, and CD40) were measured by flow cytometry. DC-TSL expressed the highest level of HLA-DR and CD86. DC-ITC also exhibited elevated HLA-DR expression, but expressed the lowest level of co-stimulatory molecules. HLA-DR expression on the surface of DC-TCSs remained at the same level as in unpulsed DCs. Data shown are representative of three experimentsBack to article page