Fig. 5

Induction of TAA-specific CTL activity by three DC Ag-loading strategies. a Ag-loaded DCs were used as stimulators of autologous PBLs to compare their efficacy in CTL stimulation against Ag-pulsed target cells. PBLs and DCs were generated from a single donor. Data are from one representative experiment. b TAA-specific cytotoxicity was determined by flow cytometry. Data are the mean percentages of cytotoxicity ± SD of triplicate determinations. TCL-DCs induced a more potent CTL response compared with DC-ITC as shown by the increased cytotoxicity from SNU899-derived Ag-pulsed autologous DCs used as targets at a 10:1 E:T. Statistical comparisons were performed using Student’s t-test