Fig. 3

Smad4 is a downstream target of miR-130a-3p. a Left panel: Real-time RT-PCR assay was used to detect the mRNA level of Smad4 in HCC GR cells. *p < 0.05 vs control. Right panel: Western blotting analysis was performed to measure the expression of Smad4 in HCC GR cells. b Left panel: Sequences of target sites for miR-130a-3p in Smad4 are shown. Right panel: Luciferase reporter assays were performed to identify the binding of miR-130a-3p to Smad4 3′-UTR. WT: wild type; Mut: mutation. *p < 0.05 vs control