Fig. 5

Down-regulation of Smad4 inhibited cell migration and invasion in HCC GR cells. a Top panel: Transfection of HepG2 GR cells and SMMC-7721 GR cells with Smad4 siRNA inhibited cell migration. Bottom panel: Smad4 siRNA suppressed cell invasion of HepG2 GR and SMMC-7721 GR cells by Transwell invasion assay. b Quantitative results are illustrated for panel a. c SMMC-7721 GR cells transfected with Smad4 siRNA caused decreased motility capacity as assessed by Wound healing assay. d HepG2 GR and SMMC-7721 GR cells transfected with Smad4 siRNA inhibited the attachment and detachment of cells. * P < 0.05 vs control siRNA