Fig. 4

SOCS3 induces pancreatic cancer cell apoptosis and cell cycle inhibition. a Protein level of SOCS3 was effectively enhanced after transfection with expression vector in Bxpc3, and SOCS3 knockdown in Panc1 was efficient after transfection with siSOCS3 1# compared to siNC. b The CCK8 assay was used to examine cell growth of SOCS3 gene stably transfected Bxpc3 cells and a reduction of absorbance was observed at 24 h, 48 h and 72 h (P < 0.01). c Panc1 and Bxpc3 cells were transiently transfected with SOCS3 siRNA and SOCS3 expression vector, respectively. Cell apoptosis was measured using the flow cytometry 48 h post transfection. d The cell cycle distribution of SOCS3-modulated Panc1 and Bxpc3 cells was analyzed using the flow cytometry. e The proportions of apoptosis cells and cells in different phases of cell cycle were quantified. Data represents three independent experiments in triplicate. f Protein expression levels of cell apoptosis and cell cycle related genes were analyzed in Bxpc3 and Panc1 cells 48 h after transfection using western blots