Table 1 PCR primers
Gene | Sequence (5′-3′) | Experimental use |
|---|---|---|
SOCS3 | CAGCTCCAAGAGCGAGTACCA (forward) | Real-time PCR |
AGAAGCCGCTCTCCTGCAG (reverse) | ||
β-ACTIN | GCACCACACCTTCTACAATGAGC (forward) | Real-time PCR |
TAGCACAGCCTGGATAGCAACG (reverse) | ||
SOCS3(M) | TGATTAAATATTATAAGAAGGTCGGTCG (forward) | Methylation-specific PCR |
ACTAACTACGTACGAAACCGAAACG (reverse) | ||
SOCS3(U) | GTAGTGATTAAATATTATAAGAAGGTTGGTTG (forward) | Methylation-specific PCR |
CTAACTACATACAAAACCAAAACAA (reverse) | ||
SOCS3 siRNA-1 | CCAAGAACCUGCGCAUCCAdTdT | RNA interference |
SOCS3 siRNA-2 | GGACCAAGAACCUACGCAUdTdT | RNA interference |
SOCS3 siRNA-3 | CCAAGAGAGCUUACUACAUdTdT | RNA interference |
SOCS3 siRNA-NC | UUCUCCGAACGUGUCACGUTT | RNA interference |