Fig. 4

TGF-β1-driven EMT is associated to enhancement of GrB expression. CRC cells were induced to undergo EMT by their incubation with TGF-β1 different time points; cells treated (TGF-β1) or untreated (Untd) with TGF-β1 were analyzed for (a) invasion through Matrigel at 24 h (*p < 0.0001) and (b) GrB expression and 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. Representative experiments out of three