Fig. 5

GrB depletion downmodulates TGF-β1-driven EMT in CRC cells. First, we knocked-down GrB in GrB positive HT-29 cells, then, we incubated cells with TGF-β1 and analyzed EMT and invasion. a GrB depletion was verified by WB in CRC cells treated or not, as indicated; EMT biomarkers (Snail 1, and E- and N-cadherin) were analyzed by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b HT-29 cell invasion through Matrigel was performed using cells transfected with GrB-specific (siGrB) or Control (siCtr) Stealth RNAi and then treated or not with TGF-β1; *p < 0.0001. Representative experiments out of three