Fig. 6

DHA inhibits GrB expression, EMT and invasion in CRC cells. GrB positive CRC cells were treated with 100 μM DHA dissolved in ethanol solution or with ethanol solution alone (EtOH) for 24 h, and (a) GrB expression and 3 EMT biomarkers (Snail 1, and E- and N-cadherin) were analyzed by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software; (b) invasion was assessed in the invasive HCT 116, HCT-8 cell lines and in CSC4; p < 0.0001. Representative experiments out of at least three