Fig. 2

Knockdown of LRG1 repressed the invasion capacity of CRC cells. a RT-PCR and Western blot analyses showed effective downregulation of LRG1 following siRNA transfection. b HCT116 and SW480 cells transfected with LRG1 siRNA or control siRNA were seeded in the upper chamber. After 48 h, the cells invaded through the membrane were stained and counted in five random microscopic fields (200×). c LRG1- and control-siRNA transfected HCT116 and SW480 cells were wounded with pipette and wound closure percentage was quantified 48 h after scratch relative to that at 0 h. *P < 0.05 compared to the control cells. Scale bar represents 100 μm