Fig. 4

LRG1 promoted VEGF-A expression and angiogenesis. a HCT116 and SW480 cells were stimulated with rLRG1 (0–1000 ng/ml) for 24 h, and VEGF-A expression was quantified by RT-PCR and ELISA. b CRC cells were stimulated with rLRG1 (500 ng/ml) for indicated hours, and VEGF-A expression was quantified by RT-PCR and ELISA. c and d The medium of HCT116 cells incubated in the presence or absence of LRG1 (500 ng/ml) and LRG1 antibody (10 μg/ml) was collected and then applied to HUVECs for transwell migration assay and tube formation assay. e Effect of CM from LRG1-induced HCT116 cells on the aortic ring sprouting assay. Representative photos were shown and data were summarized from 3 independent experiments. *Compared to the control group, P < 0.05. Scale bar represents 100 μm