Fig. 1
Expression levels of UHRF1 mRNA and protein in PCa cell lines. a Expression of UHRF1 mRNA in LNCaP, 22RV1, DU145, and PC-3 cells. b, c Western blot examination of UHRF1 protein levels in LNCaP, 22RV1, DU145, and PC-3 cells. d, g Expression of UHRF1 mRNA after transfection with the indicated siRNAs in LNCaP and PC-3cells. e, f Western blot analysis of UHRF1 protein levels in LNCaP cells 24 h after transfection. h, i Western blot analysis of UHRF1 protein levels in PC-3 cells 24 h after transfection. j Expression of UHRF1 mRNA after transfection with expression plasmid pcDNA 3.1(+)-UHRF1 in LNCaP and PC-3cells. k, l Western blot analysis of UHRF1 protein levels in PC-3 cells 24 h after transfection. Parts c, f, i and l show the relative grey values of each band (normalized to β-actin). Protein bands from three independent Western blot assays were quantified using Quantity One software (Bio-Rad, USA). Data are reported as the mean ± SD (** p < 0.01, Student’s t-test)