Fig. 3From: UHRF1 overexpression is involved in cell proliferation and biochemical recurrence in prostate cancer after radical prostatectomyUHRF1 influenced cell cycle status in PCa cells. Cells were transfected with siUHRF1-535, siUHRF1-1453, siNC, pcDNA3.1(+) or pcDNA3.1(+)-UHRF1 plasmid for 48Â h, stained with propidium iodide (PI), and used for cell cycle analysis. Treatment with siUHRF1 decreased the percentage of cells in G1 phase and increased the percentage in S phase in LNCaP (a) and PC-3 (b) cells. Overexpression of UHRF1 induced the decrease in G1 phase and the increase in S phase of LNCaP cells (c). Each experiment was performed in triplicateBack to article page