Fig. 4From: UHRF1 overexpression is involved in cell proliferation and biochemical recurrence in prostate cancer after radical prostatectomyUHRF1 inhibited prostate cancer cell apoptosis. Cells were transfected with siUHRF1-535, siUHRF1-1453, siNC, pcDNA3.1(+) or pcDNA3.1(+)-UHRF1 plasmid for 48Â h, and then subjected to cell apoptosis (stained with PI and FITC-Annexin V). Knockdown of UHRF1 caused an increase in early and late apoptotic cells and a decrease in living cells in LNCaP (a, b), 22RV1 (c, d) and PC-3 (e, f). Overexpression of UHRF1 decreased the fraction of apoptotic cells in LNCaP cells (g, h). Each experiment was performed in triplicateBack to article page