Fig. 3

PA28α knockdown in CAL27 and HSC3 inhibits cell growth, but doesn’t induce apoptosis in vitro. a PA28α was knocked down by siRNA targeting PA28α, illustrated by Western blotting. b MTT assay, PA28α silencing in CAL27 and HSC3 inhibits cell growth. c Clonogenic assay, PA28α silencing in CAL27 and HSC3 inhibits clonogenic. d Edu assay, PA28α silencing in CAL27 and HSC3 inhibits proliferation. e TUNEL assay, PA28α silencing in CAL27 and HSC3 has no effect on apoptosis. PA28α overexpression in OSCC cell lines promotes cell proliferation in vitro was indicated in Additional file 8: Figure S5. Data are reported as means ± SEM for three independantly experiments. (ANOVA or T test, *p < 0.05, ** p < 0.01, *** p < 0.001)