Fig. 2

Migration and invasion. a, HT29 cells treated with 160 μM or 320 μM AqB013 had a significantly reduced migration compared to vehicle control. b, HCT-116 cells treated with up to 320 μM AqB013 showed no significant effect on wound closure. c, the effect of AqB013 on HT29 cell invasion (n = 3) measured by an increase in sphere size (%) relative to vehicle control: in spheres treated with 80 μM or 160 μM of AqB013 there was a significant decrease in invasion at 144 hours compared with vehicle. d, HCT-116 cells treated with up to 320 μM AqB013 (n = 3) showed no significant effect on invasion. ** p = 0.004; ***p < 0.001 ****p < 0.0001. e, AqB013 treatment up to 320 μM had no effect on cell proliferation of HCT-116, at 160 μM AqB013 HT29 showed 17% reduced proliferation (*p = 0.03 ANOVA). Proliferation measured in absorbance units at 490 nm. The error bars show standard error of the mean