Fig. 3

KDM5B knockdown inhibits HCC cellular proliferation and colony formation in vitro. a and b Knockdown of endogenous KDM5B suppresses the proliferation of Hep-3B and Focus cell lines. The silencing efficiency of two siRNA (si-370, si-2531) against endogenous KDM5B was evaluated by real-time PCR, where si-NC was used as a negative control. c KDM5B RNAi suppressed colony formation in Hep-3B and Focus cells, as shown by representative plates of cells transfected with the KDM5B shRNA constructs and shRNA-NC control. The histograms represent mean with standard deviation of colonies from triplicate tests. d KDM5B RNAi limited colony formation of Hep-3B and Focus cells in soft agar. The histograms represent mean with standard deviation of colonies from triplicate tests. Here all experiments were performed independently 3 times. A t test was used to evaluate the statistical significance of these experiments, as compared to the control. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Original magnification, ×40