Fig. 7

Regulation of the p15 and p27 promoter occupancy on their promoters region by KDM5B. a and b The KDM5B occupancy and trimethylation status of histone H3 (H3K4Me3) at the promoters of p15 and p27 genes. C and R: Control siRNA and KDM5B siRNA, respectively. ChIP was used to precipitate KDM5B and H3K4Me3-bound DNA followed by PCR amplification with the specific primers spanning p15 and p27 promoters. Input: 2 % of total chromatin materials were PCR amplified with the same primers