Fig. 3
From: Anti-tumor effects of Atractylenolide I on bladder cancer cells
ATR-1 induces apoptosis in bladder cancer cells. a Representative flow cytometry profiles of apoptosis induced by different concentrations of ATR-1 in 253J (upper) and T-24 (lower) cells. b Quantitative results obtained using Annexin V/PI staining. c Western blot analysis of apoptosis-related caspases proteins, β-Actin was used as an equal loading control. d Pretreatment of Z-LEHD-FMK, and Z-DEVE-FMK inhibits the apoptosis triggered by ATR-1. Data represent mean ± S.D. (n =3).*p < 0.05, **p < 0.01