Fig. 5
From: Anti-tumor effects of Atractylenolide I on bladder cancer cells
ATR-1 suppressed PI3K/AKT/mTOR signaling pathway. a Suppression of phosphorylated AKTSer473, mTOR and induction of PTEN by ATR-1 in 253J (left) and T-24 (right) cells. β-Actin was used as an equal loading control. b Analysis of cells transfected with AKT1 constitutively active plasmid or EGFP plasmid (control). Constitutively active AKT1 inhibited the ATR-1 induced up-regulation of Bax and Bad. c & d Introduction of constitutively active AKT1 significantly blocks the anti-proliferation activity of ATR-1. Data represent mean ± S.D. (n =3).*p < 0.05, **p < 0.01