Fig. 10

Control vector (pcDNA plasmid and retroviral vector [RV]) and mesothelin overexpressing tumors harvested from mice (Fig. 6) were frozen, fixed, and stained with biotin conjugated rat anti-mouse CD8, Vectastain Elite ABC and developed with Vector DAB Peroxidase substrate. Spleen cells (positive control) show strong staining of CD8+ cells compared to negative control (no primary antibody) or tumor tissue sections