Fig. 4

Overexpression of mesothelin. a pcDNA- Clones of Panc02 cells (clones 2, 14 and 17) stably transfected with pcDNA mesothelin under the CMV promoter show higher levels of mesothelin expression than wild type Panc02 cells and empty pcDNA vector Panc02 cells. HEK293 cells transiently transfected with empty vector or mesothelin expressing pcDNA vector (293Meso) are shown as control. b Overexpression of mesothelin is seen in stably transduced retrovirus (RV) clones of Panc02 (clones, 2, 4, and 10), compared to retrovirus vector. HEK 293 cells transiently transfected with mesothelin over-expressing plasmid or empty vector are shown as controls