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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer

Fig. 5

Mesothelin expression on the cell surface. Panc02 wild type (wt), retrovirus (RV), clones 2, 4, 10; or pcDNA clones 2, 14, and 17, or control null vector cells were incubated with monoclonal antibody B35 (black line) or normal rat IgG (gray line) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Mean fluorescence intensities (MFI) show increased mesothelin in overexpressing clones. RV clones expressed significantly more mesothelin than vector and wild type p < 0.001. pcDNA clones 2 and 14 expressed significantly more mesothelin than vector and wild type p < 0.05

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