Fig. 7

Mesothelin effect on in vitro growth. Wild type (WT) Panc02, mesothelin over-expressing stable Panc02 cells constructed using retrovirus (RV, clones 2, 4, 10) or pcDNA transfection (clones 2, 14, and 17) or control null vector containing Panc02 cells were seeded in triplicate in 96-well plates (15,000 cells/100ul/well) with 10 % serum. After 24 h, 10 μl of MTS/PMS was added to measure metabolism/proliferation, and the absorbance was read at 492 nm