Fig. 3
NHPI induces G2/M phase cell cycle arrest by inhibiting the expressions of cyclin B1 and cdc2. a BT-20 cells were treated with NHPI at 2.5, 5 and 10 μM for 24 h. LoVo cells were incubated with NHPI at 5, 10 and 15 μM for 24 h. Cells were stained with PI and subjected to flow cytometry analysis. Representative images were shown. b Quantitative analysis of cells in each cell cycle phase was performed. Results were presented as mean ± SD (n = 3). *p < 0.05 and ***p <0.001, difference versus 0 μM control group. c The inhibition of cyclin B1 and cdc2 expressions contributed to NHPI-induced G2/M phase arrest. Cells were treated with indicated concentrations of NHPI for 24 h, and the expression levels of G2/M phase regulatory proteins were analyzed by western blot analysis. β-actin was used as a loading control