Fig. 5

NHPI inhibits both mTORC1 and mTORC2 signaling in BT-20 and LoVo cells. a BT-20, LoVo and MDA-MB-231 cells were treated with indicated concentrations of NHPI for 24 h, followed by western blot analysis with indicated antibodies. β-actin was used as a loading control. b BT-20 and LoVo cells were treated with indicated concentrations of NHPI for 24 h, followed by western blot analysis with indicated antibodies. β-actin was used as a loading control. c NHPI induced the eIF4E nuclear translocation in BT-20 cells. BT-20 cells were incubated with indicated concentrations of NHPI for 6 h. Then the cells were analyzed by immunofluorescence assay labeling with eIF4E antibody and DAPI. eIF4E and nucleus were recognized by the red and blue fluorescence, respectively