Fig. 2

The expression of CCR7 in different GBC cell lines after treatment with TNF-α. a, d Expression of CCR7 in three human GBC cell lines (NOZ, GBC-SD, and SGC-996) detected by Western blotting. The levels of CCR7 protein in NOZ and SGC-996 cells were higher when compared to the GBC-SD cell lines. b, e GBC cells (NOZ and SGC-996) were treated with varying concentrations of TNF-α (5, 10, 20 and 50 ng⁄mL) for 12 h. The CCR7 protein level was measured by Western-blotting; the level increased in a dose-dependent manner. c, f CCR7 protein in NOZ and SGC-996 cells was detected after treatment with 20 ng/mL TNF-α for 6, 12, and 24 h. (*P < 0.05)