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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: CCR7 mediates the TNF-α-induced lymphatic metastasis of gallbladder cancer through the “ERK1/2 - AP-1” and “JNK - AP-1” pathways

Fig. 4

Expression of CCR7 in NOZ cells using RNAi technology. a Three siRNA oligos targeting the CCR7 gene (si1, si2 and si3) and a non-targeting control (siNC) were designed and chemically synthesized, and then transiently transfected into the NOZ cells. b, c The CCR7 protein in NOZ cells transfected with siRNA oligos was detected by Western blotting. The relative expression of CCR7 in the cells transfected with si3 oligos was significantly reduced compared to the negative control and siNC groups (*P < 0.05). d Construction of a NOZ cell line stably expressing lentiviral CCR7 (producing si3 sequence) or NC shRNA and a green fluorescent protein sequence. The cells were observed under a fluorescence microscope with blue light. e The CCR7 expression of NOZ cells stably transfected with LV-shCCR7 and LV-shNC was analyzed by western blotting. The protein level of CCR7 in the LV-shCCR7 group was significantly decreased relative to the LV-shNC group (*P < 0.05)

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