Fig. 1

SOX2 was regulated by EWS/FLI1 and highly expressed in Ewing’s sarcoma. a, and b Expression of SOX2 was significantly decreased by EWS/FLI1 knockdown in A673 and RD-ES cells in terms of both on mRNA and protein levels. Data are representative of three independent experiments. c SOX2 was expressed in Ewing’s sarcoma but not in normal tissues around bones examined by WB analysis. d Representative images of IHC staining of SOX2 in normal tissues and Ewing’s sarcoma. All data are presented as means ± S.D. **P < 0.001