Fig. 6

SOX2 inhibition suppressed Ewing’s sarcoma growth in vivo. a Tumor growth was significantly repressed by siSOX2 intratumoral injection as shown in the curve graph (Data are presented as the mean ± S.D. **P < 0.001). b SOX2 inhibition substantially decreased xenograft tumor volume, shown as representative images exhibited. c Representative images of IHC analysis demonstrated the significant repression of SOX2, p-Akt and cyclin-E, and the enhancement of cleaved-caspase-3 in tumor samples treated with siSOX2. d Variations of the upstream regulators, such as SOX2, PI3K, Akt and p-Akt, the downstream anti-survival of proteins, such as Fas, caspase-8, Bad, caspase-9, caspase-3, p21 and p27, and the pro-proliferation factors, such as PARP and cyclin-E, were all followed the same trends described as in vitro, as analyzed by WB. The data are representative of three independent experiments