Fig. 4

Peptide R effect on macrophages/microglial cells accumulation in U87MG glioma. a Representative CLSM analyses of brain sections of vehicle-treated (CTRL), peptide R- or Plerixafor-treated mice stained with anti-CD11b (red) and CD68 (green, a marker for activated microglia) antibodies. Arrows indicate the tumor edge, while asterisks (*) indicate the tumor-free parenchyma. Scale bars, 30 μm. b Mean intensity of CD11b and CD68 fluorescence were analysed by Image J software and reported as mean values ± SD (n = 4–5 images for each treatment group). *P = 0.0420 and **P = 0.0066 (asterisks shown in figure), one-way ANOVA. P = 0.0476 and P = 0.0056 (peptide R versus CTRL), unpaired two-tailed Student t-test