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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Low dose of lenalidmide and PI3K/mTOR inhibitor trigger synergistic cytoxicity in activated B cell-like subtype of diffuse large B cell lymphoma

Fig. 4

The role of NF-κB in apoptosis induced by NVP-Bez235 and lenalidomide. a Cell lines were exposed to 5nM bortezomib for 24 h and 48 h, respectively. Cell lysis were conducted with immunoblotting for p-NF-κB and NF-κB. b Bar graph was represented as mean ± S.D (*p < 0.05) to show the cell viability of cell lines treated with 5nM bortezomib and concomitant treatmemt of NVP-Bez235 (20nM) and lenalidomide (2 μM). c All cell lines were transiently transfected with siRNA against NF-κB (si-NF-κB) and nonsilencing siRNA (si-NS) respectively for 24 h and then exposed to NVP-Bez235 (20nM) plus lenalidomide (2 μM) for 48 h. Then cells were collected for Western blot. d Cells were transfected as (c) for 24 h and treated with compounds for another 48 h. Then CCK-8 assay was performed to calculate the cell viability. *p < 0.05, **p < 0.01, ***p < 0.005 compared with si-NS group

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