Fig. 2

Number and origin of MPs by flow cytometry analyses in study subjects. A representative set of scattergrams in two samples from colon cancer patients is shown to illustrate MPs and subpopulation definitions. Lactadherin-positive MPs were examined for expression of other antigens by co-labeling with Alexa Fluor 488- and Alexa Fluor 647-labeled antibodies as is shown here for platelet-derived MPs (Alexa Fluro 488-CD41a⁺) and endothelia-derived MPs (Alexa Fluro 647-CD31⁺/Alexa Fluor 488-CD41a⁻) in (a) stage II or (b) IV of colon cancer patients. Comparison of c PS⁺ total MPs, d platelet-derived MPs (PMPs), e endothelial-derived MPs (EMPs), and f tissue factor-positive MPs (TF⁺ MPs) among healthy subjects (n = 33), stage I (n = 16), II (n = 47), III (n = 28), or IV (n = 21) colon cancer patients. Data are given as median (horizontal bar), 25th and 75th percentile (boxes), and 10th and 90th percentile (error bar). *P <0.05; **P <0.01; ***P <0.001