Fig. 5

PS exposure on platelets and MPs and fibrin formation was evaluated using fluorescence confocal microscopy. a Platelets from healthy subjects barely marked with green fluorescence, b while platelets from colon cancer stage IV were labeled with lactadherin (green fluorescence, arrowhead) and PMPs (triangles) were shedding from PS exposed platelets. c MPs were strongly labeled with both Alexa 488-lactadherin (green) and Alexa 647-annexin V (red) indicating extensive PS exposure. d Fibrin labeled with red fluorescence was observed along with PS⁺ MPs labeled with lactadherin (green). Representative image; scale bars represent 10 μm (b) or 5 μm (c and d)