Fig. 1

dsP53-285 induces wild-type p53 expression by targeting promoter in human bladder cancer cells. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs or mock transfection for 72 h. GAPDH levels were detected and served as a loading control. a Expression of p53 mRNA levels was assessed by real-time PCR. b Expression of p53 protein was detected by Western blot analysis. c Expression of p21 mRNA was detected by real-time PCR. d Expression of p21 protein was detected by Western blot analysis. ^* P < 0.05, ^** P < 0.01 and ^*** P < 0.001 compared to mock and dsControl groups. (e and f) Time-course of dsP53-285-mediated upregulation of p53 expression. Cells were subjected to real-time PCR or western blot at the indicated time points. ^* P < 0.01 and ^** P < 0.001 compared to 0 h; ^# P < 0.05 and ^## P < 0.01 compared to 24 h; ^& P < 0.05 compared to 48 h