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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Quercetin sensitizes glioblastoma to t-AUCB by dual inhibition of Hsp27 and COX-2 in vitro and in vivo

Fig. 4

t-AUCB increases COX-2 expression by Hsp27 activation. 200 μM t-AUCB treatment increases COX-2 mRNA expression (a) (**P < 0.01) and protein expression (b). c: t-AUCB-induced COX-2 overexpression and Hsp27 phosphorylation was partially attenuated by SB203580. d: Hsp27 protein expression and its phosphorylation and COX-2 expression level were suppressed by quercetin or Hsp27 siRNA in cells without t-AUCB treatment. In cells treated with quercetin plus t-AUCB, decrease of COX-2 and Hsp27 expression and Hsp27 phosphorylation was partially reversed. β-actin served as loading control. The protein intensity of the immunoblotting band was quantified by ImageJ and represented by the relative value compare with loading control (1.00)

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