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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell

Fig. 2

a MBIC induced apoptosis in HeLa cells: Flow cytometry analysis of HeLa cells treated with various concentration of MBIC for 24 h was carried out. Representative figures show population of viable cells in Q3 (annexin V- PI-), early apoptotic cells in Q4 (annexin V+ PI-), late apoptotic cells in Q2 (annexin V+ PI+) and necrotic cells in Q1 (annexin V- PI+). Representative figure shows apoptosis induction of MBIC (0.21, 0.42 and 1 μM) against HeLa cells 24 h after treatment. b shows early and late induced apoptosis in bar chart for HeLa cells 24 h after treatment. Data were mean ± SD of three independent experiments. All the treatment groups were compared with control. “*” indicates statistically significant at P < 0.05. c MBIC induced G2-M arrest in HeLa cells: HeLa cells were treated with indicated concentrations of MBIC (0.21, 0.42 and 1 μM) for 24 h. Cells were permeabilized by ethanol and stained with PI. Cell cycle progression has been assessed by flow cytometry. Representative figures of cell cycle distribution (G0-G1, S, and G2-M) show accumulation of MBIC-treated cells in G2-M phase. HeLa cells were arrested in G2-M phase 24 h after MBIC treatment

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