Fig. 4

Validation of gene expression profile for outcome prediction. a Unsupervised hierarchical clustering analysis applied to 43 patients according to the expression of our 7 genes signature for prognosis prediction. Expression of selected 7 prognostic relevant genes determined by RT-PCR was used to cluster all 43 patients included in the study. Unsupervised cluster distinguished 2 groups of samples (red, bad prognosis; and green, good prognosis). b Expression of 7 genes for prognosis prediction in the 2 clustered groups (red and green bars in Figs. 1a and 4a) determined by RT-PCR. Results were normalized against the expression level of GAPDH. High expression of ID3, IGJ, ID1 and CMTM8 was shown to be associated with predicted poor prognosis (PPP)