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Table 1 TOP1/CEN-20 FISH analysis

From: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations

Cell line TOP1 CEN-20 TOP1/CEN-20 ratio Description of aberrationa
HCT116-Wt 2 2 1 None
HCT116-SN38 A: 2, B: 3 A: 2, B: 2 A: 1, B: 1.5 Two subpopulations; A (46 %): No aberration, B (54 %): TOP1 gain
HT29-Wt 5 4 1.25 20q isochromosome formation
HT29-SN38 4 4 1 20q isochromosome formation, loss of chromosome 20 and gain of CEN-20 on chromosome without TOP1
LoVo-Wt 2 2 1 None
LoVo-SN38 2 2 1 None
  1. Wt (wild-type) designates the parental cell lines
  2. aFor details see text