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Table 1 TOP1/CEN-20 FISH analysis

From: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations

Cell line

TOP1

CEN-20

TOP1/CEN-20 ratio

Description of aberrationa

HCT116-Wt

2

2

1

None

HCT116-SN38

A: 2, B: 3

A: 2, B: 2

A: 1, B: 1.5

Two subpopulations; A (46 %): No aberration, B (54 %): TOP1 gain

HT29-Wt

5

4

1.25

20q isochromosome formation

HT29-SN38

4

4

1

20q isochromosome formation, loss of chromosome 20 and gain of CEN-20 on chromosome without TOP1

LoVo-Wt

2

2

1

None

LoVo-SN38

2

2

1

None

  1. Wt (wild-type) designates the parental cell lines
  2. aFor details see text